Figure 3.

Evolutionary relationship of 100 representative bacterial isolates (taxa) based on the 16S rDNA gene sequences. Each representative was selected for their uniqueness in terms of N-acyl homoserine lactone (AHL) production and/or quenching properties. The evolutionary relationship was inferred using the neighbour-joining method. The bootstrap consensus tree inferred from 1,000 replicates was taken to represent the evolutionary relationship of the taxa analysed. Branches corresponding to partitions reproduced in <50% bootstrap replicates were collapsed. The tree was drawn to scale, with branch lengths reflecting evolutionary distances. The evolutionary distances were computed using the maximum composite likelihood method and are in the units of the number of base substitutions per site. All positions containing gaps and missing data were eliminated from the data set (complete deletion option). There were a total of 1,031 positions in the final data set. Phylogenetic analyses were conducted in MEGA4. The outgroups, retrieved from the GenBank database, are underlined. The isolated strains are classified as producer (green triangle), quencher (blue square), producer and quencher (red diamond) and neither producer nor quencher (open circle). The genera in which AHL production (@) or AHL degradation (*) activities have been reported in the literature are indicated. The closest relative with at least 97% sequence identity for each strain, except for those strains highlighted in bold, is shown.