Figure 4.

A comparison between the signalling properties of the floccular (left panel) and the granular (right panel) sludge communities. The relationships between the abundance of the top 50 most dominant community members (each represented by a unique V6 tag) and the concentration profile of N-acyl homoserine lactones (AHLs) over time were calculated based on Pearson correlation for the respective floccular and granular sludge communities. The Pearson correlation coefficient is colour coded, indicating relationships from strongly positive (+1, green) to strongly negative (−1, red) interactions. ^▲Unclassified based on the taxonomy classification pipeline stated in Materials and methods section. The closest relative with sequence identity matching >97% is shown where it is applicable. False discovery rate corrections for multiple comparisons were performed, and significant differences are indicated as follows: *P<0.05, **P<0.01 and ***P<0.001. The signalling properties of the granular sludge community was adapted from Tan et al.¹⁹