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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: Transl Res. 2017 May 18;185:13–23. doi: 10.1016/j.trsl.2017.05.003

Figure 4. β-END release from scratch wounded keratinocytes.

Figure 4

(A) The opioid peptide, β-END, released from HaCaT keratinocytes was measured in culture medium from monolayers at unwounded (UW), wounded (W) and 0.5, 1, 2, 4, and 24 hours after wounding. (B) HDMECs proliferation was assessed by cell proliferation ELISA after treatment with conditioned medium from wounded HaCaT cell medium. Significance was determined by t-test (A, unpaired, two-tailed) or one-way ANOVA (B). Abbreviations: MOPr, mu-opioid receptor; WT, wild-type; β-END, beta-endorphin; SF, serum free; UW, unwounded; W, wounded; KC, keratinocytes; HaCaT, human keratinocytes; HDMECs, Human dermal microvascular endothelial cells; CTOP, D-Phe-Cys-Tyr-D-Trp-Orn-Thr-Pen-Thr-NH2.