REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-AID	(Basu et al., 2011)	N/A
Goat polyclonal anti AID	Novusbio	NB100-93454
Anti-β actin	Sigma-Aldrich	A1978
Anti-DNA/RNA hybrid (S9.6)	Kerafast	ENH001
Anti-Exosc3	Genway	GWB-FF795C
Anti Exosc3	Santa Cruz Biotechnology	Sc-98776
Anti-SKIV2L2 (Mtr4)	Abcam	ab70551
B220-PE	Biolegend	103208
IgG1-FITC	BD Bioscience	553443
IgA-FITC	BD Bioscience	559354
Anti-Flag M2 magnetic beads for IP	Sigma-Aldrich	M8823
Anti-Flag M2-HRP for WB	Sigma-Aldrich	A8592
Chicken polyclonal anti Flag-tag	Abcam	Ab1170
Alexa488 (Against-Goat)	Jackson Immuno Research	805-547-008
Alexa Fluor 647 (Anti-Rabbit)	Jackson Immuno Research	711-605-152
Goat antibody, ATTO 488 conjugated	Rockland Immunochemical	610-152-041
Nuclease-Free Water	Qiagen	129115
Chemicals, Peptides, and Recombinant Proteins
LPS	Sigma-Aldrich	L4130
IL4	Peprotech	214-14
TGFβ	R&D	240-B-010
cOmplete ™	Roche	11836170001
Tris base	Fisher	BP152-1
Streptavidin-HRP	Thermo Scientific	PI-21130
VPD450	BD Bioscience	562158
Q5 HF-Taq	NEB	M0491S
NaCl	Sigma	S5150-1L
HA-peptide	Fisher	26184
Flag-peptide	Sigma	F3290-4MG
M-280 Streptavidin Dynabeads	Life Technologies	11205D
Protein G Dynabeads	Life Technologies	10003D
Protein A/G agarose	Pierce	20423
Anti-HA agarose	Fisher	26181
M2-agarose anti-FLAG resin	Sigma	A22220-1ml
Glycerol	Sigma	G5516-1L
Dithiothreitol (DTT)	Gold Biotech	DTT25
MOPS	Sigma	M1442-500ml
Polyethylenimine (PEI)	Fisher	NC9197339
Cystamine	Sigma	30070-10G
Protease Inhibitor Cocktail tab.	Sigma	11-836-170-001
Dulbecco’s Modified Eagle Medium (DMEM)	Life Technology	10565018
Paraformaldehyde	Electron Microscopy Sciences	15710
Glutaraldehyde	Fisher	50-262-10
Normal goat serum(NGS)	Jackson Immuno Research Laboratories, Inc.	005-000-121
RiboLock	Fisher	EO0381
MgCl2	Sigma	M1028
MOPS buffer	Sigma	M1442
Critical Commercial Assays
Ion Xpress™ Plus gDNA Fragment Library Preparation	Life Technologies	4471269
Ion Shear™ Plus Reagents Kit	Life Technologies	4471248
Ion Plus Fragment Library Kit	Life Technologies	4471252
CD43 magnetic beads	Miltenyi biotec	130-049-801
Mouse B Cell Nucleofector Kit	Lonza	VPA-1010
Deposited Data
RNA-seq data	(Pefanis et al., 2014; Pefanis et al., 2015)	SRP042355
Experimental Models: Cell Lines
CH12F3	(Nakamura et al., 1996)	N/A
129S6/SvEvTac X C57BL/6Tac hybrid mESC	Columbia U. transgenic facility	N/A
HEK293T	ATCC	ATCC-CRL-3216
Experimental Models: Organisms/Strains
Exosc3TAPtag/TAPtag mouse	This paper	N/A
Exosc10COIN/COIN mouse	(Pefanis et al., 2015)	N/A
Rosa26 CreERt2 mouse	Gift from Dr. Piere Chambon (IGBMC)	N/A
Rosa26BirA/BirA mouse	(Driegen et al., 2005)	N/A
Recombinant DNA
BAC clone	Source Bioscience	bMQ386a13
pSpCas9(BB)-2A-GFP (pX458)	Addgene	48138
pGEM-T Easy	Promega	A1360
pcDNA3.1	Fisher	V79020
Sequence-Based Reagents
Primers for sanger sequencing (see table S2)	This paper	N/A
Primers for NGS (see table S2)	This paper	N/A
Primers for DRIP (see table S2)	This paper	N/A
Primers for RT-qPCR (see table S2)	This paper	N/A
Sequences of AID gRNA: TGAGACCTACCTCTGCTACG	This paper	N/A
Sequences for Setx gRNA: AAGCGCTATGCATCTAGCAC	This paper	N/A
Sequences for Mtr4 gRNA: AATCAGAGTCAGCCAGTGGG and AAATGTGGCCCCAGTTGCCC	This paper	N/A
Sequences for Ung gRNA: CGCAACGTGCCTGCCGGCTT and TCACGGACGCGGTCGTGTCC	This paper	N/A
Primers for sub cloning of hMtr4 in to pCDNA3.1- reverse primer: 5′ACGTCAGCTAGCCTATAA ATA CAA AGA3′	This paper	N/A
Primers for sub cloning of hAID in to pCDNA3.1- forward primer: 5′GTGGTCGCTAGCATG TACCCATACGATGTT CCAGATTACGCTATG GACAGCCTCTTG3′	This paper	N/A
Primers for sub cloning of hAID in to pCDNA3.1- reverse primer: 5′ACGTCAGCTAGCTCA AAG TCCCAAAGT3′	This paper	N/A
Primers for unwinding assay for Mtr4- R16- Top strand-reporter: 5′AGCACCGUAAAGACGC3′	This paper	N/A
Primers for unwinding assay for Mtr4- R22- Bottom strand: 5′GCGUCUUUACGGUGCUUAAAAA3′	This paper	N/A
Primers for unwinding assay for Mtr4- D22- Bottom strand: 5′GCGTCTTTACGGTGCTTAAAAA3′	This paper	N/A
Software and Algorithms
BWA-MEM. arXiv:1303.3997v1 [q-bio.GN]		http://github.com/lh3/bwa
Bowtie2	(Langmead and Salzberg, 2012)	http://bowtie-bio.sourceforge.net/bowtie2/in dex.shtml
Samtools		http://samtools.sourceforge.net/
MATLAB		https://www.mathworks.com
Rstudio		https://www.rstudio.com/
R		https://www.r-project.org/
NIS element	NIKON	N/A
FlowJo	BD Bioscience	N/A
Other
Bladder, Liver and Lung mutational exosome data		gdac.broadinstitute.org
Multiple Myeloma	(Lohr et al., 2014)	http://www.ncbi.nlm.nih.gov/pubmed/24434212
Illustration image	Servier Medical Art	http://www.servier.com/Powerpoint-image-bank