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. 2016 Jan 27;2:15027. doi: 10.1038/npjbiofilms.2015.27

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Copyright © 2016 Nanyang Technological University/Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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B. subtilis engulfment of and invasion into B. simplex biofilm mediates eradication of B. simplex. (a) Percentage of B. subtilis and B. simplex colony forming units at each stage of the interaction: the interacting colonies were divided into three sections: B. sub. area—B. subtilis section, Int.—interaction zone and B. sim area—B. simplex section. Each section was collected, sonicated and plated to determine the number of replicative cells of each species. In each section, percentage of each species from total cell number is presented (n=6). (b) Sum of the cell numbers from all sections as described in a, during 4 days of interaction (n=4). (c) Cell number from all sections as described in a, during 4 days of interaction (n=4). Error bars represent s.d.