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. 2016 Jan 27;2:15027. doi: 10.1038/npjbiofilms.2015.27

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Copyright © 2016 Nanyang Technological University/Macmillan Publishers Limited

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Loss of the natural B. subtilis plasmid increases the production of B. subtilis killing factors that inhibit rival Bacillus species growth (a) (i) B. subtilis plasmid-cured strain. (ii) B. subtilis plasmid-cured strains isolated from interacting B. subtilis-B. simplex biofilms. (iii, iv) Genetic Complimentation with the rapP for the strains in (i) and (ii) respectively. (b) Percentage of B. subtilis biofilms cells that lost their natural plasmid during the interaction with B. simplex. n=6, error bars represent s.d. (c) The role of RapP in regulation of the killing factors that inhibit rival Bacillus species growth. (d, e) Growth curves of Bacillus species exposed to supernatant of B. subtilis WT, plasmid-cured, and interaction evolved strain. Bacteria were grown in 96-well plates, with shaking, at 30 °C. (b) B. simplex growth curves. MSgg medium supplemented with 8-h ×0.25 B. subtilis supernatants. n=6, error bars represent s.d. (d) Growth curves of B. toyonensis. Liquid LB broth medium, supplemented with 8-h ×0.25 B. subtilis supernatants. n=6, error bars represent the s.d.