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. 2016 Mar 2;2:15031. doi: 10.1038/npjbiofilms.2015.31

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Copyright © 2016 Nanyang Technological University/Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Biomineralization is required for formation of complex morphology in Mycobacterium smegmatis. (a, b) Upper panel: top view of the biofilm of the undomesticated M. smegmatis. The biofilms were grown on solid biomineralization-promoting medium without (a) or with (a, b) a calcium source, for 3 (a), 5, 21 (b) days at 30 °C, in a CO₂-enriched environment. Images were taken with a Stereo microscope with an objective ×1. Scale bar corresponds to 500 μm. Lower panel: MicroCT images of the biofilms formed by M. smegmatis. Scale bar corresponds to 2 mm. The results are of a representative experiment out of three independent repeats. (c) The FTIR spectra of calcium carbonate minerals precipitated within the colony. The designation of the peaks as v₂, v₃ and v₄ is marked. The results are of a representative experiment out of five independent repeats.