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. 2016 Jun 15;2:16002. doi: 10.1038/npjbiofilms.2016.2

Figure 3.

Figure 3

The effect of degradation intermediates of PHB on the phenotypic expression of virulence factors. (A) The effect of PHB on the motility behaviour of Vibrio PUGSK8. The PHB treatment effected the loss of twitching, swarming and swimming behaviours. The effect of PHB on twitching (a–control, d—treated), swarming (b—control, e—treated) and swimming (c—control, f—treated). (B) CLSM images shows the effect PHB on biofilm formation by Vibrio PUGSK8. The a is control and b, c and d is showing the effect of PHB on biofilm formation at concentrations of 50, 100 and 150 μg/ml, respectively. (C) Haemolytic activity of Vibrio PUGSK8 on blood agar plate. (a) The cell-free supernatant (CFS) of untreated Vibrio PUGSK8 showing haemolytic activity (b) Haemolytic activity of CFS collected from PHB-treated Vibrio (24 h) (c) Complete inactivation of haemolytic activity of CFS collected from PHB-treated Vibrio (48 h).