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. 2016 Jun 15;2:16002. doi: 10.1038/npjbiofilms.2016.2

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Copyright © 2016 Published in partnership with the Nanyang Technological University

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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GC-MS analysis of AHL signalling molecules produced by Vibrio PUGSK8. (a) is showing GC-MS characteristic peaks of standard AHL. (b) Characteristic peaks of AHL produced by Vibrio PUGSK8 evidences the cell signalling molecule as N-hexanoyl homoserine lactone. (c) Absence of characteristic GC-MS peaks of AHL indicating the PHB shuts off PHB mediated cell signalling in Vibrio. (d). The fragmentation pattern elucidated based on the MS spectra of AHL produced by Vibrio PUGSK8 showed conformity with Cataldi et al. (2008).