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. 2016 Jun 8;2:16008. doi: 10.1038/npjbiofilms.2016.8

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Copyright © 2016 Nanyang Technological University/Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Representative confocal laser scanning microscopy images of cryoconite sediment with associated microbial communities and biofilm. (a) Green=SYBR Green stained microbes, grey=reflection of the sediment. (b,c) Red=auto-fluorescencing cells, green=SYBR Green stained microbes, grey=reflection of the sediment. (d) Blue=Calcofluor White stained EPS, grey=reflection of the sediment. (e) Red=Propidium Iodide (membrane compromised cells) Green=Syto9 stain (live cells). (f) Control image of combusted cryoconite sediment following described staining protocol, grey=reflection of the sediment.