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. 2016 Jun 8;2:16008. doi: 10.1038/npjbiofilms.2016.8

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Copyright © 2016 Nanyang Technological University/Macmillan Publishers Limited

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Example of nanoSIMS isotope ratio images of an analysed Oscillatoria sp. filament for a the ¹³C/¹²C ratio (b) the ¹⁵N/¹⁴N ratio (c) the epifluorescence overlay used to confirm cell identification of hybridised Bacteroidetes cells (green), DAPI stained (blue) and an autofluorescent filament (red). White lines indicate regions of interest (ROIs) section of an analysed Oscillatoria sp. filament. (d) NanoSIMS analysis of ¹³C and ¹⁵N enrichment measurements atom % (AT%) for Bacteroidetes sp. cells based on proximity to filamentous Oscillatoria, and Oscillatoria cells (▼). Cells <2 μm from a filamentous cell (●), and cells >2 μm from a filamentous cell (▲).