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. Author manuscript; available in PMC: 2018 Jun 5.
Published in final edited form as: Dev Cell. 2017 Jun 5;41(5):540–554.e7. doi: 10.1016/j.devcel.2017.05.007

Figure 6. Nup210 and Mef2C Regulate a Common Set of Muscle Genes.

Figure 6

(A) Whole-genome gene expression was analyzed by microarray in C2C12 myotubes infected with lentiviruses carrying control, Nup210, or Mef2C shRNAs. Heatmap shows the gene expression patterns of Nup210- and Mef2C-depleted myotubes (2,410 common genes, p ≤ 0.05).

(B) Scatterplots of log2 gene expression changes in Nup210- and Mef2C-depleted myotubes (p ≤ 0.05). Genes changing ≥ 1.25-fold for Nup210 and Mef2C are shown in blue (down) or red (up). Gene expression values are mean changes of microarray sextuplicate samples.

(C) Venn diagram of down- and upregulated genes in Nup210- and Mef2C-depleted myotubes relative to scramble control (p ≤ 0.05, ≥ 1.25-fold change).

(D) Process enrichment for Nup210 and Mef2C co-regulated genes was analyzed using Metacore software.

(E) Heatmap for gene expression changes of sarcomeric, cell adhesion, and muscle contraction genes in Nup210- and Mef2C-depleted myotubes.

(F) The expression levels of Myl2, Tmod1, miR-133a-1, and miR-206 in Nup210 and Mef2C-depleted myotubes were analyzed by qPCR. mRNA levels for each gene were normalized to Hprt1 expression and are presented relative to scramble control-treated cells. Data represent mean ± SEM, n ≥ 3 replicates.

See also Figures S3 and S4.