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. Author manuscript; available in PMC: 2018 Jun 5.
Published in final edited form as: Dev Cell. 2017 Jun 5;41(5):540–554.e7. doi: 10.1016/j.devcel.2017.05.007

Figure 8. Nup210 Is Required for the Recruitment of Mef2C to Its Target Genes but Is Dispensable for Their Association with NPCs.

Figure 8

(A) DNA-FISH for Nup210 target genes was performed on quiescent myoblasts. Gene loci (red), Lamin A (green). Representative images of four independent experiments.

(B) DNA-FISH was performed on control C2C12 myotubes or myotubes depleted of Nup210. Left panel shows that Nup210 is efficiently downregulated by the specific shRNA in myotubes. Right panel: gene loci (red), Lamin A (green). Representative images of two independent experiments.

(C) ChIPs were performed in control or Nup210-depleted differentiated C2C12 myotubes using a Mef2C-specific antibody and analyzed by real-time PCR against the specified genes. Values represent the relative fold enrichment of knockdown cells to control cells.

(D) ChIPs were performed in control or Nup210-depleted differentiated C2C12 myotubes using an H3K27Ac-specific antibody by real-time PCR against the specified genes. Values represent the relative fold enrichment of knockdown cells to control cells.

(E) Schematic model of Nup210 regulation of muscle gene expression during myoblast differentiation. Undifferentiated myoblasts do not express Nup210. When cells are induced to differentiate, Nup210 is added to NPCs and recruits Mef2C through Trip6. The assembled complex regulates the expression of local muscle genes involved in sarcomere assembly, myofiber maturation, and muscle growth.

Bar plots represent mean ± SEM, n ≥ 3 replicates. Scale bars, 5 µm. See also Figure S8.