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. 2017 May 31;67(3):268–276. doi: 10.1270/jsbbs.16200

Fig. 4.

Fig. 4

Comparison of DNA sequencing chromatograms of RsRBCS2B during two-step TILLING. In the BC1M1 population (line 27), a G-to-A point mutation was detected at nucleotide 134 from ATG, which causes the amino acid substitution S45F. Direct sequencing chromatograms using DNA from wild type (A), a bulk BC1M1 population with a wild-type: mutated sequences ratio of 3:1 (or less) (B), a heterozygous mutant individual BC1M1 plant (C), and a selected homozygous mutant from a BC1M2 population (D) are shown.