Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Nov 2;2:16033. doi: 10.1038/npjbcancer.2016.33

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016 The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

M2-polarized macrophages sustain tumor aggressiveness and influence drug sensitivity of AXL-overexpressing breast cancer cells. (a) Wound-healing assays were conducted with untreated or R428-treated MDA-MB-231 cells cultivated in the absence (control medium) or presence of conditioned medium (CM) derived from in vitro polarized M2 macrophages. (b) Statistical analysis of wound closure. Gap size at 0 h was set to 100% and percentage of closed wound was calculated after 24 h. (c) M2-polarized macrophages increase the resistance of HCC38 and MDA-MB-231 cells to paclitaxel (Pac) and doxorubicin (Dox) treatments compared with cells treated with control medium, whereas the selective inhibition of AXL with R428 restores drug sensitivity in TNBC cells. (d) Western blot with the indicated antibodies of lysates from MDA-MB-231 cells treated with 1 μmol/l R428 at different time points. β-Actin was used as a loading control. All P values were obtained using a two-tailed Student’s t-test (mean±s.d., N=3 experiments; *P<0.05; **P⩽0.01).