Figure 7.

Induction of gene expression by cytokines of the alternative and classical groups. MPs derived from PBMC from five normal donors were stimulated for 4 h with four cytokines/soluble factors selected from the alternative group (IL-13, IL-1α, IL-4, TGF-β) and four from the classical group (FLT-3 L, IFN-γ, IL-3, CD40L). Gene expression was assessed by TaqMan real-time PCR. Relative estimates of (a) mannose receptor, (b) NFκB and (c) TRAF binding protein genes were calculated by averaging the ratio of the quantity mean of each gene normalized to the reference AFAP gene in five donors. Statistical significance is expressed as Student's t-test p-value.