Table 1.
Concentration and doses of cytokines/soluble factors used for stimulation of monocytes
| Cytokine | Concentration in vitro | Source |
| Aldara | 3 μM | 3M Pharmaceuticals |
| BCA-1 | 10 ng/ml | Peprotech |
| CD40 L | 500 ng/ml | Peprotech |
| FLT-3 ligand | 100 ng/ml | Peprotech |
| GM-CSF | 1,000 IU/ml | Peprotech |
| IFN-γ | 1,000 U/ml | Biogen |
| IFN-α2 | 1,000 U/ml | Peprotech |
| IFN-α2b (α2, α2b,) | 1,000 U/ml | PBL Biomedical Laboratories |
| IFN-αA (2a) | 1,000 U/ml | PBL Biomedical Laboratories |
| IFN-αI | 1,000 U/ml | PBL Biomedical Laboratories |
| IFN-αB2 | 1,000 U/ml | PBL Biomedical Laboratorios |
| IFN-α4b | 1,000 U/ml | PBL Biomedical Laboratories |
| IFN-αC | 1,000 U/ml | PBL Biomedical Laboratories |
| IFN-αF | 1,000 U/ml | PBL Biomedical Laboratories |
| IFN-αG | 1,000 U/ml | PBL Biomedical Laboratories |
| IFN-αH2 | 1,000 U/ml | PBL Biomedical Laboratories |
| IFN-αJ1 | 1,000 U/ml | PBL Biomedical Laboratories |
| IFN-αK | 1,000 U/ml | PBL Biomedical Laboratories |
| IFN-αWA | 1,000 U/ml | PBL Biomedical Laboratories |
| IFN-β | 1,000 U/ml | PBL Biomedical Laboratories |
| IL-1α | 10 ng/ml | National Cancer Institute (NCI) Biological Research Branch |
| IL-1β | 10 ng/ml | NCI Biological Research Branch |
| IL-2 | 6,000 IU/ml | Chiron |
| IL-3 | 15 ng/ml | NCI Biological Research Branch |
| IL-4 | 1,000 IU/ml | Peprotech |
| IL-5 | 10 ng/ml | Peprotech |
| IL-6 | 100 ng/ml | Peprotech |
| IL-8 | 100 ng/ml | Peprotech |
| IL-10 | 10 ng/ml | Peprotech |
| IL-12 | 10 ng/ml | Peprotech |
| IL-13 | 20 ng/ml | Peprotech |
| IL-15 | 20 ng/ml | Peprotech |
| LPS only | 5 μg/ml | Sigma Aldrich |
| MIP-1α (CCL3) | 100 ng/ml | Peprotech |
| MIP-4 (PARC, CCl18) | 100 ng/ml | Peprotech |
| MIP-1β (CCL4) | 100 ng/ml | Peprotech |
| RANTES (CCL5) | 100 ng/ml | Peprotech |
| TARC (CCL17) | 100 ng/ml | Peprotech |
| TGF-α | 10 ng/ml | Peprotech |
| TNF-β | 20 ng/ml | Peprotech |
| TGF-β | 5 ng/ml | Peprotech |
| TNF-α | 100 ng/ml | Peprotech |
| VEGF | 10 ng/ml | R&D Systems |