. Author manuscript; available in PMC: 2018 May 4.

Published in final edited form as: Nanoscale. 2017 May 4;9(17):5664–5670. doi: 10.1039/c7nr01369h

Table 1.

Comparison of annealing and melting rates of two reporter strands at different Tris-HCl and glycerol concentration

reporter	sequence (5′ to 3′)	Tris-HCl buffer	glycerol conc.	k_on (10⁶M⁻¹s⁻¹)	k_off (s⁻¹)
reporter strand-1	ATTO633-TGGGCGGG	20 mM	70%	5.13±0.40	9.55±0.64
		4 mM	70%	3.63±0.66	21.60±0.40
		20 mM	80%	0.45±0.05	10.99±2.0

reporter strand-2	ATTO633-TGATTGTG	20 mM	70%	5.65±0.56	15.71±1.98