Table 2. Primary antibodies.
Antibody | Company | Reference | Host | Dilution IF | Dilution WB |
---|---|---|---|---|---|
Anti-CK14 | Covance | PRB-155P | Rabbit | 1:500 | 1:2000 |
Anti-CK5 | Abcam | ab53121 | Rabbit | 1:100 | 1:500 |
Anti-CK8 | Home made | TROMA-1 | Rat | 1:200 | 1:1000 |
Anti-Epcam | Abcam | ab32392 | Rabbit | 1:100 | 1:2500 |
Anti-FN | Thermo Scientific | MA1-12597 | Mouse | 1:100 | |
Anti-FN | Abcam | ab299 | Rabbit | 1:4000 | |
Anti-Ki67 | Thermo Scientific | RM-9106 | Rabbit | 1:200 | |
Anti-Nestin | Millipore | MAB5326 | Mouse | 1:300 | |
Anti-NSE | Dako | M0873 | Mouse | 1:300 | |
Anti-p63 | DAKO | M7317 | Mouse | 1:100 | |
Anti-p63 | Abcam | ab124762 | Rabbit | 1:1000 | |
Anti-S100 | DAKO | Z0311 | Rabbit | 1:500 | |
Anti-S100β | Sigma | S2532 | Mouse | 1:100 | |
Anti-Synapto | Abcam | ab16659 | Rabbit | 1:100 | |
Anti-SMA | Sigma-Aldrich | A5228 | Mouse | 1:1000 | |
Anti-Tuj1 | Covance | PRB-435P | Rabbit | 1:1000 | 1:5000 |
Anti-Vimentin | Bioss | bs-0756R | Rabbit | 1:300 | |
Anti-Vinculin | Sigma-Aldrich | WH0007414M1 | Mouse | 1:5000 |