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. 2017 Jul 18;12(7):e0181591. doi: 10.1371/journal.pone.0181591

Fig 2. Identification of two fusion transcripts.

Fig 2

Total RNA isolated from the heads of one-day-old heterozygotes with the paternal transmission of the inverted allele were used for detecting two fusion transcripts. (right panel) Two sets of primers were used for this RT-PCR-based experiments: the first set of three primers (p1,p3,p9) target the exons of Peg3 whereas the second set of three primers (u1,u3,u9) target the exons of Usp29. The relative orientations and positions of these primers are indicated with arrows underneath the exon structures of Peg3 and Usp29. (left panel) A set of cDNA prepared from the KO and WT of both sexes with the paternal transmission of the inverted allele were tested with various primer sets: including an internal control (β-actin), Peg3 sets (p1-p3, p1-p9), Usp29 sets (u1-u3, u1-u9), U-Peg3 sets (u1-p3, u1-p9) and P-Usp29 sets (p1-u3, p1-u9).