Fig 3. Effects of the inversion on the expression levels of the Peg3 domain.

Potential effects of the inversion on the expression levels of the individual genes within the Peg3 domain were analyzed using a set of total RNA isolated from the heterozygotes of both sexes with the paternal transmission of the inverted allele. (A) This series of qRT-PCR analyses used several primer sets targeting the four imprinted genes, including Peg3, Usp29, Zim1 and Zfp264. In the case of the Peg3 and Usp29, the expression levels of both the fusion and original transcripts were simultaneously measured through two sets of primers targeting the shared exons between the fusion and original transcripts. (B) The expression levels of each gene were first normalized with an internal control (β-actin), and the subsequent value was compared between KO versus WT. The relative levels are presented in a graph with standard errors (S.E.). This set of analyses were repeated using three biological replicates.