Fig 5. AFM force mapping.
AFM topography maps of cross-linker-free microgel particles pOEGMA80/0 (a) and 5 mol-% PEG-DA cross-linked particles pOEGMA80/5 (b) after rehydration in 25 mM HEPES/150mM NaCl, pH 7.4. Each image has a scan size of 2 μm x 2 μm. For every square a complete force-distance curve was collected. The grids placed on top of the particles indicate the squares used for analysis. Particle heights and the corresponding Young´s moduli are plotted versus the particle cross section of microgels pOEGMA80/0 (c) and pOEGMA80/5 (d).