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. 2017 Jul 3;13(7):e1006868. doi: 10.1371/journal.pgen.1006868

Fig 9. Tc-zld knockdown in larval stages affects elytra and wings in adult stage.

Fig 9

(A) Control elytron extracted from a dsneo adult shows a parallel venation pattern and rigid chitinous structure. (B) Tc-zld RNAi elytron displays a disrupted vein pattern and a less resistant structure. (C,D) Hindwings were dissected and photographed. Although the overall morphological pattern is not affected in both wings, zld dsRNA wings show severe dehydration after ethanol fixation. (E) PD indexes comparison between dsneo and dszld RNAi hindwings and forewings, a PD index reflects the shape of the wing based on its dimensions ratio [52]. The statistical analysis was carried out by unpaired t-test assuming unequal variances (asterisks refer to P<0.0001 while ns stands for “no significance”), indicating that Tc-zelda RNAi affects hindwing shape. (F) Tc-zld RNAi elytra and hindwing do not show statistically significant differences in length when compared to their respective dsneo RNAi controls.