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. 2017 Jul 18;12(7):e0181382. doi: 10.1371/journal.pone.0181382

Fig 3. Proliferative capacity of long-term vaccine-elicited human T cells.

Fig 3

Cryopreserved and thawed PBMCs from pre-vaccination (Pre), 6 months (6m), and 1 (1y) and 2 (2y) years after the last vaccine administration were labelled with CFSE, stimulated with pools of stimulatory 15-mer peptides assembled for each returning volunteer for 5 days and analyzed using flow cytometry for dividing (decreased CFSE) CD4+ and CD8+ T cells. The CM and DDDCM vaccination regimens are indicated above. The gating strategy is given in S1 Fig. Individual values are shown with median as a horizontal line.