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. 2017 Jul 18;12(7):e0181382. doi: 10.1371/journal.pone.0181382

Fig 5. Dextramer-aided analysis of human YV9- and KV10-specific CD8+ T cells.

Fig 5

For HLA-A*02:01-positive vaccine recipients, frozen and thawed PBMCs from 6 months (6m), and 1 (1y) and 2 (2y) years after vaccination were incubated with HLA-A*02:01-YV9 (A and C) or HLA-A*02:01-KV10 (B and D) dextramers together with other cell-surface markers and analyzed using flow cytometry (S3 Fig). Alternatively, PBMCs of subject 416 were stimulated with personalized 15-mer peptide pools and reacted with a panel of functional mAbs together with HLA-A*02:01-YV9 (E and F) or HLA-A*02:01-KV10 (H and I) dextramers. The pie charts (F and I) show the plurifunctionality of dextramer positive CD8+ T cells. PBMCs from subject 416 reactive with the HLA-A*02:01-YV9 (G) or HLA-A*02:01-KV10 (J) dextramers were phenotyped for memory subsets defined as TN−naïve T cells (CD45RAhiCCR7hiCD27hi). TCM—central memory (CD45RAloCCR7hiCD27hi), TTM—transitional memory (CD45RAloCCR7loCD27hi), TEM—effector memory (CD45RAloCCR7loCD27lo), and TTD—terminally differentiated (CD45RAhiCCR7loCD27lo) (S3 Fig).