Table 2.

The amino acids in the peptide clusters for T Antigen stimulation of PBMCs

		JCV T Antigen Peptide Clusters
Cluster	AA	Sequence		Target
C1	1–51	mdkvlnrees melmdllgld rsawgnipvm rkaylkkcke lhpdkggded k	POL α
C2	41 – 91	lhpdkggded kmkrmnflyk kmeqgvkvah qpdfgtwnss evptygtdew e	POL α
C3	81 – 131	evptygtdew eswwntfnek wdedlfchee mfasddentg sqhstppkkk k	pRB	p107	Phosphorylation site
C4	121 – 171	sqhstppkkk kkvedpkdfp vdlhaflsqa vfsnrtvasf avyttkekaq i		Helicase	DNA binding NLS
C5	161 – 211	avyttkekaq ilykklmeky svtfisrhgf gghnilfflt phrhrvsain n		Helicase	DNA binding
C6	201 – 251	phrhrvsain nycqklctfs flickgvnke ylfysalcrq pyavveesiq		Helicase	DNA binding
C7	241 – 291	pyavveesiq gglkehdfnp eepeetkqvs wklvtqyale tkcedvfllm g	POL α	Helicase	DNA binding Zinc finger
C8	281 – 331	tkcedvfllm gmyldfqenp qqckkcekkd qpnhfnhhek hyynaqifad s	POL α	Helicase	Zinc finger
C9	321 – 371	hyynaqifad sknqksicqq avdtvaakqr vdsihmtree mlverfnfll d	POL α	Helicase	Zinc finger
C10	361 – 411	mlverfnfll dkmdlifgah gnavleqyma gvawihcllp qmdtviydfl k	POL α	Helicase
C11	401 – 451	qmdtviydfl kcivlnipkk rywlfkgpid sgkttlaaal ldlcggksln v	POL α	Helicase	ATP binding. ATPase
C12	441 – 491	ldlcggksln vnmplerlnf elgvgidqfm vvfedvkgtg aesrdlpsgh g	POL α	Helicase	ATP binding. ATPase
C13	481 – 531	aesrdlpsgh gisnldclrd yldgsvkvnl erkhqnkrtq vfppgivtmn e	POL α	Helicase	ATP binding. ATPase
C14	521 – 571	vfppgivtmn eysvprtlqa rfvrqidfrp kaylrkslsc seyllekril q		Helicase; p53	ATP binding. ATPase
C15	561 – 611	seyllekril qsgmtlllll iwfrpvadfa aaiherivqw kerldleism y		Helicase; p53	ATP binding. ATPase
C16	601 – 651	kerldleism ytfstmkanv gmgrpildfp reedseaeds ghgsstesqs q		Helicase; p53	ATP binding. ATPase
C17	641 – 688	ghgsstesqs qcfsqvseas gadtqenctf hickgfqcfk kpktpppk	p53	Host range	Phosphorylation site

AA, amino acid; POL α, DNA polymerase α; NLS, nuclear localization signal.