Table 1. List of 27 genes analyzed in the retina by qRT-PCR for expression level.
| CONTROL | RAD | IRON | IRON+RAD |
Main effect |
|||
|---|---|---|---|---|---|---|---|
| RAD | IRON | IRON+RAD | |||||
|
Apoptosis related | |||||||
| ATG12 (autophagy-related protein 12) | 84.58±33.00 | 108.41±28.60 | 52.56±8.83 | 67.55±14.14 | P=0.007 | P=0.08 | |
| Bcl2 (B-cell lymphoma 2) | 3.19±2.00 | 2.44±0.82 | 1.79±0.59 | 1.50±0.25 | P=0.02 | ||
| Casp1 (caspase 1) | 1.45±0.00 | 1.28±0.31 | 1.59±0.47 | 1.38±0.24 | |||
| Casp2 (caspase 2) | 13.38±7.00 | 15.60±4.50 | 6.06±3.34 | 10.35±2.26 | P=0.04 | P=0.09 | |
| Casp3 (caspase 3) | 3.65±2.00 | 4.83±2.12 | 2.14±1.19 | 2.19±0.44 | P=0.02 | ||
| CLU (clusterin) | 728.83±254.00 | 789.68±206.88 | 601.21±196.35 | 589.38±193.74 | |||
| HiF1 (hypoxia-indicible factor) | 265.43±193.00 | 443.87±331.98 | 183.64±45.33 | 185.27±113.26 | P=0.09 | ||
|
Iron metabolism | |||||||
| Cp1 (ceruloplasmin) | 109.19±48.00 | 95.35±51.05 | 70.17±8.27 | 92.02±46.88 | |||
| FtH1 (Ferritin, Heavy) | 2.58±0.58 | 2.89±0.53 | 2.36±0.39 | 2.82±1.31 | |||
| FtL1 (Ferritin, Light) | 57.44±45.00 | 101.68±73.90 | 183.05±173.98 | 280.95±226.71 | P=0.09 | ||
| Tf1 (transferrin 1) | 3.21±1.14 | 3.26±2.32 | 2.40±7.34 | 3.55±2.04 | |||
|
Cellular growth | |||||||
| Egr1 (early growth response factor) | 28.26±33.00 | 20.44±23.24 | 28.41±32.77 | 15.02±8.19 | |||
| CFH (complement factor H) | 61.74±26.00 | 73.44±17.29 | 34.92±4.25 | 71.75±30.97 | P=0.02 | ||
| TrkA (tyrosine kinase receptor type 1) | 13.10±7.00 | 7.46±4.48 | 6.41±3.26 | 10.21±5.72 | P=0.10 | ||
| VEGF (vascular endothelial growth factor) | 6.67±5.00 | 9.21±8.63 | 3.49±0.76 | 4.94±2.08 | |||
|
Eye growth and function | |||||||
| Gfap (glial fibrillary acidic protein) | 79.18±32.00 | 145.09±69.76 | 72.33±23.63 | 175.19±184.27 | P=0.05 | ||
| Gndf (glial cell line derived neurotrophic factor) | 1.40±0.00 | 1.69±0.86 | 1.69±0.52 | 1.38±0.24 | |||
| Opn4 (melanopsin pigment) | 14.56±6.00 | 31.96±15.98 | 13.58±8.22 | 14.26±1.93 | P=0.08 | P=0.07 | |
| Rho (rhodopsin pigment) | 10.86±13.00 | 9.54±8.78 | 10.39±8.71 | 31.45±54.70 | |||
|
Cellular stress response | |||||||
| Hmox1 (heme oxygenase 1) | 7.08±2.39 | 4.67±1.32 | 8.08±1.31 | 14.70±2.20 | P=0.001 | ||
| Nrf2 (nuclear factor like 2) | 14.33±8.21 | 14.81±11.15 | 5.72±2.18 | 16.61±5.39 | P=0.05 | ||
| HSF1 (heat shock factor) | 50.57±15.93 | 53.66±17.22 | 25.50±4.36 | 28.23±7.22 | P=0.001 | ||
| HSP27 (heat shock factor 27) | 188.86±182.80 | 193.51±204.24 | 489.15±213.76 | 276.19±235.35 | |||
| HSP70 (heat shock factor 70) | 1.40±0.36 | 1.76±0.85 | 1.59±0.47 | 1.45±0.24 | P=0.02 | ||
| Sirt1 (Sirtuin 1) | 26.21±12.16 | 31.42±10.57 | 15.14±4.68 | 18.66±3.54 | |||
| SOD2 (Mitochondrial superoxide dismutase) | 168.39±83.41 | 152.27±113.83 | 100.78±22.94 | 230.01±177.47 | |||
Abbreviations: ANOVA, analysis of variance; ECM, extracellular matrix; IRON, high-iron diet/no radiation; IRON+RAD, high-iron diet/radiation; qRT-PCR, quantitative reverse transcription PCR; RAD, adequate-iron diet/radiation.
Expression level of 27 genes analyzed in the retina by qRT-PCR. The [(2−Δct)×1000] values for all genes investigated in the retina are listed. P values are based on a two-way ANOVA of the treatment effects calculated using Δct values.