Table 1.

List of ligands and the corresponding mechanical types.

Ligand	Mechanical responsea	Unloaded MT off-rate, (koff0, s−1)	Detachment Force (Fc, pN)	Class
Control	–	0.35	17	–
Monastrol	Weak-binding	0.92	27	Less potent L5
Ispinesib	Weak-binding	1.50	18	Less potent L5
STLC	Weak-binding	1.45	0.6	Potent L5
Filanesib (ARRY-520)	Weak-binding	1.74	2.4	Potent L5
BRD9876	Strong-binding	0.01b	14	Rigor

^aMechanical classification according to Figure 1, 2, and Figure S1.

^bMT-unbinding rate in BRD9876 was estimated by its IC₅₀ in 3 mM ATP.