Figure 6. Hepatic cellular stress responsive pathways.
(A) Representative Western blots for phosphorylated and total forms of P38, Erk1/2 and Akt protein in liver lysates of male Ames dwarf (Prop1df/df) and Littermate control male mice (N) subjected to early-life GH or saline treatment. (B) Expression of Egr1, Fos, Fra-1 and IGF-I mRNA levels in the liver of GH treated dwarf and normal mice. Data are normalized to Gapdh values and expressed as a ratio (fold change) to the level seen in saline injected mice. Saline-treated-control mice (Black Bar), Saline-treated-dwarf mice (White Bar) and GH-treated-dwarf mice (Grey Bar), N = 8 male mice per group; *p<0.05, **p<0.01, ***p<0.001. a,b values that do not share a superscript letter are statistically significant (p<0.05).