Figure 4. H3-G34R mutants show DNA damage sensitivity upon replication stress.
(a) 5-fold serial dilutions showing the effect of methyl methanesulfonate (MMS), camptothecin (CPT), and hydroxyurea (HU) on the indicated strains. (b) Effect of γ−irradiation (IR) exposure on viability of H3-WT, H3-G34R, set2Δ, and rad51Δ cells. Data represent mean ± SEM from 2 biological replicate experiments. (c) Serial dilution assay showing the effect of bleomycin on the indicated strains. (d) Serial dilution assay showing the genotoxin sensitivity of H3-WT, H3-G34R, H3-WT set2Δ, and H3-G34R set2Δ cells upon MMS, CPT, or HU treatment. (e) Genotoxin sensitivity of indicated strains containing either an empty vector (EV) or a vector overexpressing Set2-3xFLAG (pSet2).