Appendix 1—table 1.

Parameter values.

DOI: http://dx.doi.org/10.7554/eLife.24998.031

Equation	Parameter	Value	Reference
1	Kd	1 nM	Vinod and Novak (2015)
	v	100	Estimated and validated using data from Figure 7
2	kcatMAST3	0.0988 s−1	Estimated using data from Figure 1a,b
	KmMAST3	0.09 µM	Obtained from Figure 1b
	a	0.37526	Estimated using data from Figure 1a,b
	r	1.2	Estimated using data from Figure 4b
	kcatPP2A	0.05 s−1	Vinod and Novak (2015)
3	kcatPKA	0.935 s−1	Estimated using data from Figure 1c,d
	KmPKA	1.6 µM	Obtained from Figure 1d
	b	2.36	Estimated using data from Figure 1c,d
	kcatPP1	0.5 s−1	Estimated using data from Hayer and Bhalla (2005)
	KmPP1	1 µM	Estimated using data from Hayer and Bhalla (2005)
4	kppx	0.05 s−1	Estimated in this study
	k88	0.01865 µM−1s−1	Estimated using data from Figure 1a,b
	kPKA	0.097	Estimated using data from Figure 4b
5	kcAMP	0.7 s−1	Estimated and validated by Zawadzki and Taylor (2004)
	n	2	Estimated and validated by Zawadzki and Taylor (2004)
	KA	10 µM	Estimated and validated by Zawadzki and Taylor (2004)
	k46	0.02335 µM−1.s−1	Estimated using data from Figure 1c,d