Figure 2. IgLON5 antibody cell-based assay and IgG subtypes.

IgLON5-IgG subclasses were characterized in patient serum. Goat antihuman IgGs (reactive with all IgG [A] or 1 IgG subclass [C–F]) were used as secondary antibodies. IgG reactivity was more prominent for IgG1 (C) and IgG4 (F) than for IgG2 (D) or IgG3 (E). Patient IgG was nonreactive with mock-transfected cells (B). Scale bar: 0.5 mm.