Fig. 2.

The effects of doxorubicin+10 nmol/l rapamycin on the viability of HepG2 cells cultured under (a) normoxic and (b) hypoxic conditions. HepG2 cells were seeded onto 96-well plates (1×10⁴ cells/well) and incubated overnight. Plates were then exposed to doxorubicin+10 nmol/l rapamycin and incubated for 24, 48 and 72 h under normoxic or hypoxic conditions. Cell viability was estimated using the MTS assay and normalized to untreated control. Data points represent mean±SEM from at least three independent experiments. For statistical analysis, analysis of variance was carried out. *P<0.000, **P<0.05.