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. 2005 Mar;15(3):428–435. doi: 10.1101/gr.3258105

Figure 4.

Figure 4.

Cre-mediated cassette exchange at the 4930562A09Rik locus. (A) Sequences of the LNPAT1 provirus inserted in the 4930562A09Rik locus were replaced by the ZeoPTC gene trap cassette, which was then replaced by NeoPTC. (B) Clones containing the proper gene replacements were identified by Southern blot analysis. The replacement of LNAPT1 by ZeoPTC (upper panel) was accompanied by the loss of the 4.5-kb Neo-hybridizing SacI fragment and the presence of a 3.2-kb Zeo-hybridizing fragment (lanes 4,11). SacI sites are located in each LTR and in the Tk gene. The replacement of ZeoPTC by NeoPTC (lower panel) was accompanied by the loss of the 3.2-kb Zeo-hybridizing fragment and the presence of a 3.6-kb Neo-hybridizing fragment (clones 7, 8, 9, 13, 15, 18, 19, 21, and 23).