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. 2005 Mar;15(3):428–435. doi: 10.1101/gr.3258105

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Figure 5. — Fusion gene expression in replacement clones. RNA from cell clones expressing poly(A) traps inserted in the (A) 4930562A09Rik,(B) LOC228098, and (C) 4933407O12Rik genes were analyzed by Northern blot hybridization, probing with gene-specific, Neo-specific, and Zeo-specific probes. The clones in A were obtained by replacing an LNPAT1 poly(A) trap (Pol2Neo) inserted in the 4930562A09Rik gene with ZeoPTC (PGKZeo), and then by replacing the ZeoPTC cassette with NeoPTC (PGKNeo). The clones in B were obtained by replacing a NeoPTC poly(A) trap in the LOC228098 gene with either ZeoPTC (PGKZeo) or IntZeoPTC (PGKIntZeo). The clones in C were obtained by replacing a IntZeoPTC (PGKIntZeo) poly(A) trap in the 4933407O12Rik gene with NeoPTC (PGKNeo). All clones expressed fusion transcripts of the expected size as indicated on the left. None of the targeted genes appeared to be expressed in the parental stem cells (ES), confirming the ability of poly(A) traps to disrupt nonexpressed genes.