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. 2005 Mar;15(3):428–435. doi: 10.1101/gr.3258105

Figure 6.

Figure 6.

Stability of Neo- and Zeo-4933407O12Rik fusion transcripts. (A) Northern blot analysis of RNA decay in actinomycin D-treated cells. RNAs were extracted from actinomycin D-treated cells expressing Neo-4933407O12Rik and Zeo-4933407O12Rik fusion transcripts, and the Northern blots were hybridized to a 4933407O12Rik-specific probe. RNAs were also probed with a β-actin probe to assess relative levels of RNA in each sample. (B) Kinetics of fusion transcript turnover. Relative levels of Neo-4933407O12Rik (squares) and Zeo-4933407O12Rik (circles) fusion transcripts in A were measured by PhosphorImager densitometry and plotted as a function of time. Zeo-4933407O12Rik fusion transcripts were twice as stable as the otherwise identical Neo fusion transcripts.