Skip to main content
. Author manuscript; available in PMC: 2018 Mar 28.
Published in final edited form as: ACS Nano. 2016 Sep 28;10(10):9243–9258. doi: 10.1021/acsnano.6b02776

Figure 2.

Figure 2

Stroma-vessel architecture affects the intratumoral distribution of nontargeted NPs. (A) Immunostaining of CD31 (red for blood vessels) and αSMA (green for fibroblasts) in subcutaneous tumor models (paraffin sections) with different tumor-stroma architectures. H460, 4T1, and BXPC3 belong to the stroma-vessel phenotype. Tumor nests (T) are highlighted in the images. D4M and A375lu belong to tumor- vessel phenotype. (B) Confocal images of DiI-labeled NP distribution in D4M, H460, and BXPC3 8 h post-injection (cryosections). The fibroblasts were visualized by staining with αSMA. (C) Flow cytometry analysis of the association of nontargeted DiI-labeled NPs with either αSMA-positive fibroblasts or other nonlabeled cells 8 h post-injection in the four different tumors (n = 3; * P < 0.05; ** P < 0.01; *** P < 0.001).