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. Author manuscript; available in PMC: 2018 Aug 1.
Published in final edited form as: J Hepatol. 2017 Mar 22;67(2):360–369. doi: 10.1016/j.jhep.2017.03.012

Figure 3. Analysis of cell death, proliferation and ductular reaction in CON and KO on normal and high iron diet.

Figure 3

(A) Representative images of TUNEL staining shows marginal increase in cell death in CON+Fe, but a greater increase in KO+Fe. (B) Ki-67 IHC demonstrates comparable and increased number of cells in S-phase after iron overload in both CON+Fe and KO+Fe. (C) Cyclin-D1 staining is increased in midzonal hepatocytes in CON+Fe compared to CON−Fe. KO+Fe exhibit less Cyclin-D1 staining in periportal hepatocytes and in ductular cells. (D) KO+Fe have increased ductular reaction as evident by IHC for Sox9. (E) Increased ductular reaction in KO+Fe was confirmed by EpCAM staining. (F) Increased ductular reaction in KO+Fe was also confirmed by IHC for CK19. (Image magnification:100×, except EpCAM:200×).