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. Author manuscript; available in PMC: 2018 Aug 1.
Published in final edited form as: J Hepatol. 2017 Mar 22;67(2):360–369. doi: 10.1016/j.jhep.2017.03.012

Figure 4. Increased levels of inflammatory cytokines precede steatohepatitis and fibrosis in KO+Fe as shown by time course after iron-feeding.

Figure 4

(A) H&E, Sirius Red and IHC for CD45 and F480 in CON and KO livers after 4-week iron diet shows presence of steatohepatitis in KO+Fe and initiation of fibrosis. (B) H&E, Sirius Red and IHC for CD45 and F480 in CON and KO livers after 5-day iron diet. Con+FE and KO+Fe lack steatosis and fibrosis while CD45-positive inflammatory cells and F480-macrophages appear to be comparably increased in both groups. (C) Cytokine assay using liver lysates from 5-day CON+Fe and KO+Fe reveals comparable increased protein levels of CCL5, CXCL10, IL-6, TNFα, and KC/Gro in KO+Fe but no change in CCl2. *p<0.05, **p<0.01, #p<0.005, #p<0.001 using Student’s T test. (Image magnification: 100×)