Figure 1.

Identification of MRF and MEF2 targets. (A) Approach used to elucidate myogenic transcriptional networks. (MRFs) myogenic regulatory factors; (GO) Gene Ontology; (TFs) transcription factors; (PWMs) position-weight matrices. (B) Venn diagrams representing the overlap of MyoD targets in three different populations studied (left) and overlap of MyoD, myogenin, and MEF2 targets in myotubes (right). The total number of targets is indicated in parentheses. (C) ChIP assays in C2C12 myotubes with anti-MyoD and anti-myogenin antibodies, showing specific enrichment of selected target genes. (D) ChIP assays in C2C12 myotubes with anti-MEF2 and anti-myogenin antibodies. (E) Identification of MyoD targets in growing, primary human myoblasts using ChIP.