Skip to main content
. 2005 Mar 1;19(5):583–592. doi: 10.1101/gad.1269305

Figure 7.

Figure 7.

A single suppressor tRNA is active in both cytoplasmic and mitochondrial translation. (A) The yeast strain W303 (ura3-52 and trp1-1UAG) containing an empty p316(URA3+) vector (EV) or p316 with an amber suppressor mutant tRNAnGlnCUG gene (tRNAnGlnCUA) was grown on media lacking uracil (-URA) or tryptophan (-Trp). tRNAnGlnCUA-dependent suppression of the trp1-1UAG mutation is shown by the ability of W303 to grow in the absence of tryptophan. (B) Cox2p expression in wild-type yeast (WT) or cox2-114UAG (HM4) (Kolesnikova et al. 2000) visualized via immunofluorescence with an anti-Cox2p antibody. tRNAnGlnCUA-dependent suppression of the cox2-114UAG mutation is shown by the increased levels of Cox2p staining in HM4 cells containing the suppressor tRNA. Cells were costained with Mito Tracker.