FIGURE 8.

The C-terminal of VP2 (331–452aa) is essential to promote the degradation of ORAOV1 and cell apoptosis. (A) Schematics represent the genes encoding the full-length VP2 and truncated VP2 molecules (from Δ1 to Δ3). The numbers indicate the amino acid positions in the molecule. (B) Hela cells were transfected with full-length pRK5-flag-vp2 (WT), the indicated truncated vp2 or empty vectors. After 48 h of transfection, total cells were stained with Annexin PE annexin-V and examined by flow cytometry for apoptosis. (C) Cell samples in B were examined by Western Blot. (D) Percentages of the annexin-V positive cells and the ORAOV1 expression levels in (B). (E) The portion of VP2 from amino acids 331–452 is responsible for ORAOV1 degradation and VP2-induced apoptosis.