Table 3.
Replication and lethality in chickens of the H5N1 viruses after inoculated intranasallya.
| Strains | Clade | Titer (log10EID50) | Virus replication on 3 DPI (log10EID50/0.1ml)b in | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Heart | Liver | Spleen | Lung | Kidney | Brain | ||||
| GSE43 | Clade 2.3.2.1.B | 6.8 | Infectedc | 6.63 | 8.63 | 8.63 | 7.75 | 8.63 | 8.63 |
| Contactd | 6.63 | 8.63 | 8.63 | 8.00 ± 1.41 | 7.81 ± 1.15 | 7.81 ± 1.15 | |||
| CKE93 | Clade 7.2 | 7.3 | Infectedc | 3.67 | 3.00 | 3.67 | 4.00 | 3.75 | 3.67 |
| Contactd | – | – | – | – | – | – | |||
a
Four-week-old SPF chickens were inoculated intranasally (i.n.) with 103 EID50 of E43 virus, 103 EID50 of E93 virus in a volume of 100 μl, respectively; three chickens in each group were euthanized on 3 DPI, and virus titer was determined in samples of heart, liver, spleen, lung, kidney, and brain in eggs.
b
For statistical analysis, a value of 1.5 was assigned if the virus was not detected from the undiluted sample in three embryonated hen eggs. Virus titers are expressed as means standard deviation in log10EID50/0.1 ml of tissue.
c
Ducks inoculated with virus.
d
Naive contact ducks housed with those inoculated.