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. 2017 Jul 18;7:5685. doi: 10.1038/s41598-017-05864-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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C9orf72 promoter activity is widespread in inhibitory neurons. (a) Representative images of layer 5 (L5) of primary somatosensory cortex (S1, top) and primary motor cortex (M1, bottom) from C9orf72 ^LacZ/+ mice stained for NeuN (green) and β-galactosidase (β-gal; red). High magnification images show β-gal-positive puncta within NeuN-positive neurons (rightmost panels). Scale bars: 50 μm and 10 μm. (b) The percentage of neurons containing β-gal puncta in layer 2/3 and L5 of somatosensory cortex and L5 of motor cortex (S1 L2/3: 362 of 429 cells, 84.4%; S1 L5, 499 of 583 cells, 85.6%; M1 L5: 425 of 523 cells, 81.3%; n = 3 mice for each group, p = 0.1378, Chi-Square test). (c) Representative images of layer 1 (L1) of somatosensory cortex stained for NeuN (green) and β-gal (red). High magnification images showing β-gal-positive puncta within L1 neurons (rightmost panels). Scale bars: 50 μm and 5 μm. (d) The percentage of L1 neurons containing β-gal puncta (26 of 32 cells, 81.3%, n = 3 mice). (e) Representative images of L5 of motor cortex stained for parvalbumin (PV, green) and β-gal (red). High magnification images show β-gal puncta within a PV interneuron (rightmost panels). Scale bars: 50 μm and 10 μm. (f) The percentage of PV interneurons containing β-gal puncta in L5 of motor cortex and somatosensory cortex (M1 L5: 36 of 51 cells, 70.6%; S1 L5: 32 of 37 cells, 86.5%; n = 3 mice for each group, p = 0.0789, Chi-Square test).