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. 2017 Jul 18;7:5703. doi: 10.1038/s41598-017-06001-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cytosolic pH is affected by caspofungin treatment. (a) Cytosolic pH was determined in the wild-type (WT), wild-type harboring the fusion Wsc1_exMid2_cyt or Mid2_exWsc1_cyt, wsc1Δ and pde1∆pde2∆ cells expressing pHluorin grown in YPD or YPD supplemented with CAS (15 ng/ml, 2 h). Equally, exponential cultures of the WT strain expressing pHluorin were exposed to Calcofluor white in YPD (CFW, 5 μg/ml, 2 h) or glucose starvation. Data are represented as mean and SD of five independent experiments. Statistical analysis of the differences in cytosolic pH was carried out using a two-tailed, unpaired, Student’s t-test: ***p ≤ 0.001. (b) Determination of glycogen accumulation, as described in Fig. 4, in the wild-type (WT), wild-type harboring the fusion Wsc1_exMid2_cyt or Mid2_exWsc1_cyt and wsc1Δ strains grown in YPD in the presence or absence of CAS.