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. 2004 Jul 29;2004(3):150–155. doi: 10.1155/S1110724304308077

Figure 4.

Figure 4

Effects of BFA on L-leucine transport in strain MMY2. MMY2 cells were incubated on MPD broth containing SDS (3 × 10-3%) and BFA (40 μg mL-1) during 10 or 20 minutes. To eliminate BFA, cells were centrifuged and the precipitate resuspended in fresh MPD. After another 60-minute incubation, cells were again centrifuged, and each pellet was resuspended at a concentration of 5 × 108 cells mL-1 in distilled H2O. For amino acid uptake values at zero or 5 minutes, 100 μL of the suspension was mixed with 100 μL-14C-Leucine, 0.1 mmol l-1 (5 mCi mmol-1). As a control for each time, an incubation in MPD with SDS was used. Viability determinations on aliquots of each incubation gave 70% for the 10-minute incubation and 58% for the 20-minute incubation.