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. 2017 Jul 19;9:229. doi: 10.3389/fnagi.2017.00229

Figure 1.

Figure 1

Inflammatory and microglial/macrophage mRNA expression profile in c-rel−/− and wild-type (wt) mice. Real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis of pro-inflammatory cytokines, interleukin 1 beta (Il1b) and interleukin 6 (Il6; A,B), and microglial/macrophage activation markers, Fc gamma receptor III (Fcgr3; C), for M1 phenotype; mannose receptor 1 (Mrc1; D), chitinase-like 3 (Ym1; E), arginase 1 (Arg1); (F), and triggering receptor expressed on myeloid cells 2 (Trem2); (G), for M2 state in substantia nigra (SN) of 4, 13 and 18 months of age mice (n = 4–6 animals per group). Four-month-old c-rel−/− mice showed increased expression of M2c microglial marker Mrc1 and Arg1, associated with marked decrease of Ym1 transcription. At 13 months of age, c-Rel deficient mice displayed an upregulation in Il1b transcription compared to younger c-rel−/− mice, while no differences in microglial M2 markers were found in c-rel−/− mice compared to wt group. No biologically relevant variations in all the analyzed markers were evident in 18-month-old c-rel−/− mice compared to wt. Data are presented as mean ± SEM. *P < 0.05, **p < 0.01, ***p < 0.001; two-way analysis of variance (ANOVA) followed by Bonferroni test for comparison vs. 4-month-old wt mice.