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. 2017 Jul 19;8:1284. doi: 10.3389/fpls.2017.01284

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Copyright © 2017 Si, Wang, Wu, Zhao, Zhang, Huang, Cai, Zhou, Dai, Zhu and Jiang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of mechanical wounding of the local leaf on the maximal photochemical efficiency of photosystem II (Fv/Fm) and net photosynthetic rate (Pn), and the relative electrolyte conductivity (REC) and content of malondialdehyde (MDA) in the systemic leaf of wheat. The local (fourth) leaf was wounded 3 h prior to exposed to the freezing stress for 24 h. Fv/Fm and Pn of the systemic (fifth) leaf were determined as area of interest after freezing stress (A). The systemic leaves were harvested after freezing stress for measurements of REC and MDA content (B). Data are means ± SD of three different replicates. Means denoted by the same letter did not significantly differ at P < 0.05 for each analysis according to Tukey’s test.