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. 2017 Jul 19;8:1284. doi: 10.3389/fpls.2017.01284

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Copyright © 2017 Si, Wang, Wu, Zhao, Zhang, Huang, Cai, Zhou, Dai, Zhu and Jiang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Requirement of NO in wounding-induced activities of antioxidant enzymes and the expression of their corresponding genes as well as RBOH. The systemic (fifth) leaf was pre-treated with distilled water, 200 μM SNP, 200 μM L-NAME, 200 μM tungstate or 200 μM c-PTIO prior to mechanical wounding. The local and systemic leaves were harvested 3 h after wounding for analysis the activities of antioxidant enzymes and the expression levels. Data are means ± SD of three different replicates. Means denoted by the same letter did not significantly differ at P < 0.05 for each leaf according to Tukey’s test.