FIGURE 8.

Transcriptional profiling of wheat plants due to the wounding and freezing treatment. Four treatments of systemic leaves: control (ck), 3 h after wounding (wo), 24 h after freezing (fr) and 3 h wounding + 24 h freezing (wo_fr) were collected for RNA sequencing analysis. The local (fourth) leaf was wounded prior to exposed to the freezing stress for 24 h. (A) Venn diagram illustrating the number of differentially expressed genes in plants treated with wounding, freezing, and wounding/freezing. The plants were treated as described in Figure 1; (B) GO analysis was performed for up-regulated genes in systemic leaves after wounding/freezing treatment.